| Features |
| |
| Speedy isolation of total RNA from various stool specimen |
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• Combination of mechanical homogenization and chemical lysis ensures optimized RNA yield, 
including small RNA
• Isolated RNA does not show any PCR inhibition due to NucleoSpin® Inhibitor Removal Columns
• Suitable for various human and animal stool samples
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| Technology |
Silica membrane technology combined with
NucleoSpin® Bead Tubes Type A |
| Format |
Mini spin columns |
| Sample material |
Fresh or frozen stool samples
|
| Fragment size |
≥ 18 nt |
| Typical yield |
10–30 µg
|
| A260/A280 |
1.9–2.1 |
| A260/A230 |
1.8-2.5 |
| Typical RIN (RNA integrity number) |
> 7.5 |
| Elution volume |
100 µL |
| Preparation time |
70 min/10 preps |
| Binding capacity |
200 µg |
| |
|
Applications*
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• RNA isolation from stool samples
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
RNase protection assays |
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* Kits to be used for research purposes only
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Ordering information
| Product |
Preps
|
Specification |
REF |
| NucleoSpin® RNA Stool |
10
|
NucleoSpin® RNA Columns with Collection Tubes, NucleoZOL, NucleoSpin® Bead Tubes Type A, NucleoSpin® Inihibitor Removal Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), rDNase, buffers |
740130.10 |
| |
50
|
as above |
740130.50 |
| Product |
Preps
|
Specification |
REF |
| NucleoZOL |
200 mL |
NucleoZOL reagent |
740404.200 |
| NucleoSpin® Bead Tubes Type A |
50 |
2 mL tubes with 0.6-0.8 mm ceramic beads, for homogenization of soil, sediments, and stool |
740786.50 |
| MN Bead Tube Holder |
1 |
To house up to 12 bead tubes in combination with a Vortex-Genie® instrument |
740469 |
|
