Features |
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Speedy isolation of total RNA from various stool specimen |
• Combination of mechanical homogenization and chemical lysis ensures optimized RNA yield,  including small RNA • Isolated RNA does not show any PCR inhibition due to NucleoSpin® Inhibitor Removal Columns • Suitable for various human and animal stool samples
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Technology |
Silica membrane technology combined with NucleoSpin® Bead Tubes Type A |
Format |
Mini spin columns |
Sample material |
Fresh or frozen stool samples
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Fragment size |
≥ 18 nt |
Typical yield |
10–30 µg
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A260/A280 |
1.9–2.1 |
A260/A230 |
1.8-2.5 |
Typical RIN (RNA integrity number) |
> 7.5 |
Elution volume |
100 µL |
Preparation time |
70 min/10 preps |
Binding capacity |
200 µg |
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Applications*
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• RNA isolation from stool samples • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays |
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* Kits to be used for research purposes only
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Ordering information
Product |
Preps
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Specification |
REF |
NucleoSpin® RNA Stool |
10
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NucleoSpin® RNA Columns with Collection Tubes, NucleoZOL, NucleoSpin® Bead Tubes Type A, NucleoSpin® Inihibitor Removal Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), rDNase, buffers |
740130.10 |
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50
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as above |
740130.50 |
Product |
Preps
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Specification |
REF |
NucleoZOL |
200 mL |
NucleoZOL reagent |
740404.200 |
NucleoSpin® Bead Tubes Type A |
50 |
2 mL tubes with 0.6-0.8 mm ceramic beads, for homogenization of soil, sediments, and stool |
740786.50 |
MN Bead Tube Holder |
1 |
To house up to 12 bead tubes in combination with a Vortex-Genie® instrument |
740469 |
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