| Features |
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Kit for purification of high-quality DNA from gram-positive and gram-negative bacteria, yeast, and fungi
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• Suitable for a large variety of starting materials
• NucleoSpin® Bead Tubes for efficient lysis included – compatible with many disruption devices
• Fast and easy procedure – Liquid Proteinase K included, no additional enzyme required
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| Technology |
Silica-membrane technology |
| Format |
Mini spin columns |
| Sample material |
< 40 mg wet weight cell pellet
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| Fragment size |
200 bp–approx. 50 kbp |
| Typical yield |
Depends on sample type and disruption
Approx. 5-25 µg (30 mg wet weight cell pellet) |
| A260/A280 |
1.6–2.0 |
| Elution volume |
100–200 µL |
| Preparation time |
35 min/prep |
| Binding capacity |
60 µg |
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| Applications* |
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• Total DNA from microbial cultures
• Typical downstream applications: PCR, real-time PCR, southern blotting, enzymatic reactions |
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| * Kits to be used for research purposes only |
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Ordering information
| Product |
Preps
|
Specification |
REF |
| NucleoSpin® Microbial DNA |
10
|
NucleoSpin® Microbial DNA Columns, NucleoSpin® Bead Tubes Type B, Collection
Tubes (2 mL), buffers, Liquid Proteinase K |
740235.10 |
| |
50
|
as above |
740235.50 |
| |
250
|
as above |
740235.250 |
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| Product |
Pieces
|
Specification |
REF |
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NucleoSpin® Bead Tubes
Type A |
50 |
2 ml tubes with ceramic beads
(included in the NucleoSpin® Soil kit) |
740786.50 |
NucleoSpin® Bead Tubes
Type B |
50 |
2 ml tubes with glass beads
(included in the NucleoSpin® Microbial kit) |
740812.50 |
NucleoSpin® Bead Tubes
Type C |
50 |
2 mL tubes with corundum |
7408 |
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