| Features |
| |
|
Isolation of genomic DNA from insects and crustaceans
|
|
• Suitable for any insect or crustacean sample – high quality DNA from fresh,
frozen, dried or ethanol preserved specimen
• NucleoSpin® Bead Tubes for efficient lysis of an exoskeleton included –
compatible with the most common disruption devices
• Fast and convenient procedure
|
| |
|
| Technology |
Silica-membrane technology combined with NucleoSpin® Bead Tubes
Type D |
| Format |
Mini spin columns |
| Sample material |
< 40 mg fresh, frozen, dried, or ethanol preserved insect / crustacean sample
|
| Fragment size |
200 bp–approx. 50 kbp |
| Typical yield |
< 25 µg (varies by sample and disruption device) |
| A260/A280 |
1.7–1.9 |
| Elution volume |
25–200 µL |
| Preparation time |
35 min/6 prep |
| Binding capacity |
60 µg |
| |
| Applications* |
| |
• Genomic DNA from fresh, frozen, dried or ethanol preserved insect or crustacean samples
• Typical downstream applications: multiplex PCR, Southern blotting, enzymatic reactions |
| |
| * Kits to be used for research purposes only |
|
| |
| |
| |
|
cation |
REF |
| NucleoSpin® DNA Insect |
10
|
NucleoSpin® DNA Insect Columns, NucleoSpin®Bead Tubes Type D, Collection Tubes (2 mL), buffers
|
740470.10 |
| |
50
|
as above |
740470.50 |
| |
|
|
|
| Product accessories |
Pieces
|
Specification |
REF |
| MN Bead Tube Holder |
1 |
Rubber-foam adapter for processing NucleoSpin® Bead Tubes with Vortex-Genie® 2 |
740469 |
NucleoSpin® Bead Tubes
Type A |
50 |
2 mL tubes with ceramic beads for sample homogenization
(0.6–0.8 mm ceramic beads; recommended for stool, soil, and sediments)
|
740786.50 |
NucleoSpin® Bead Tubes
Type B |
50 |
2 ml tubes with glass beads for sample homogenization
(40–400 μm glass beads; recommended for bacteria)
|
740812.50 |
NucleoSpin® Bead Tubes
Type C |
50 |
2 mL tubes with corundum for sample homogenization
(1–3 mm corundum; recommended for yeast and fungi)
|
740813.50 |
NucleoSpin® Bead Tubes
Type D |
50 |
2 mL tubes with steel balls for sample homogenization
(3 mm steel balls; recommended for insects, crustaceans, lipid-rich tissue) |
740814.50 |
NucleoSpin® Bead Tubes
Type E |
50 |
2 mL tubes with steel balls and glass beads for sample homogenization
(3 mm steel balls and 40–400 μm glass beads; recommended for bacteria within insects or tissue samples) |
740815.50 |
|
