Features |
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Isolation of genomic DNA from insects and crustaceans
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• Suitable for any insect or crustacean sample – high quality DNA from fresh, frozen, dried or ethanol preserved specimen • NucleoSpin® Bead Tubes for efficient lysis of an exoskeleton included – compatible with the most common disruption devices • Fast and convenient procedure
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Technology |
Silica-membrane technology combined with NucleoSpin® Bead Tubes Type D |
Format |
Mini spin columns |
Sample material |
< 40 mg fresh, frozen, dried, or ethanol preserved insect / crustacean sample
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Fragment size |
200 bp–approx. 50 kbp |
Typical yield |
< 25 µg (varies by sample and disruption device) |
A260/A280 |
1.7–1.9 |
Elution volume |
25–200 µL |
Preparation time |
35 min/6 prep |
Binding capacity |
60 µg |
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Applications* |
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• Genomic DNA from fresh, frozen, dried or ethanol preserved insect or crustacean samples • Typical downstream applications: multiplex PCR, Southern blotting, enzymatic reactions |
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* Kits to be used for research purposes only |
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cation |
REF |
NucleoSpin® DNA Insect |
10
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NucleoSpin® DNA Insect Columns, NucleoSpin®Bead Tubes Type D, Collection Tubes (2 mL), buffers
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740470.10 |
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50
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as above |
740470.50 |
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Product accessories |
Pieces
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Specification |
REF |
MN Bead Tube Holder |
1 |
Rubber-foam adapter for processing NucleoSpin® Bead Tubes with Vortex-Genie® 2 |
740469 |
NucleoSpin® Bead Tubes Type A |
50 |
2 mL tubes with ceramic beads for sample homogenization (0.6–0.8 mm ceramic beads; recommended for stool, soil, and sediments)
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740786.50 |
NucleoSpin® Bead Tubes Type B |
50 |
2 ml tubes with glass beads for sample homogenization (40–400 μm glass beads; recommended for bacteria)
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740812.50 |
NucleoSpin® Bead Tubes Type C |
50 |
2 mL tubes with corundum for sample homogenization (1–3 mm corundum; recommended for yeast and fungi)
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740813.50 |
NucleoSpin® Bead Tubes Type D |
50 |
2 mL tubes with steel balls for sample homogenization (3 mm steel balls; recommended for insects, crustaceans, lipid-rich tissue) |
740814.50 |
NucleoSpin® Bead Tubes Type E |
50 |
2 mL tubes with steel balls and glass beads for sample homogenization (3 mm steel balls and 40–400 μm glass beads; recommended for bacteria within insects or tissue samples) |
740815.50 |
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