| Features |
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Isolation of genomic DNA from lipid-rich tissue
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• Special buffer composition for efficient removal of lipids
• NucleoSpin® Bead Tubes for efficient lysis included - compatible with the
most common disruption devices
• Fast and convenient procedure without RNA contamination
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| |
|
| Technology |
Silica-membrane technology combined with NucleoSpin® Bead Tubes
Type D |
| Format |
Mini spin columns |
| Sample material |
< 40 mg fresh or frozen, lipid-rich tissue
(e.g., brain, adipose tissue, fatty fish tissue)
|
| Fragment size |
200 bp–approx. 50 kbp |
| Typical yield |
Depends on sample type, quality, and water content |
| A260/A280 |
1.7–1.9 |
| Elution volume |
25–200 µL |
| Preparation time |
35 min/6 preps |
| Binding capacity |
60 µg |
| |
| Applications* |
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• Genomic DNA from any type of tissue with a high lipid content
• Typical downstream applications: multiplex PCR, Southern blotting, enzymatic reactions |
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| * Kits to be used for research purposes only |
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Ordering information
| Product |
Preps
|
Specification |
REF |
| NucleoSpin® DNA Lipid Tissue |
10
|
NucleoSpin® DNA Lipid Tissue Columns, NucleoSpin® Bead Tubes Type D, Collection Tubes (2 mL), buffers |
740471.10 |
| |
50
|
as above |
740471.50 |
| |
|
|
|
| Product accessories |
Pieces
|
Specification |
REF |
| MN Bead Tube Holder |
1 |
Rubber-foam adapter for processing NucleoSpin® Bead Tubes with Vortex-Genie® 2 |
740469 |
NucleoSpin® Bead Tubes
Type A |
50 |
2 mL tubes with ceramic beads for sample homogenization
(0.6–0.8 mm ceramic beads; recommended for stool, soil, and sediments)
|
740786.50 |
NucleoSpin® Bead Tubes
Type B |
50 |
2 ml tubes with glass beads for sample homogenization
(40–400 μm glass beads; recommended for bacteria)
|
740812.50 |
NucleoSpin® Bead Tubes
Type C |
50 |
2 mL tubes with corundum for sample homogenization
(1–3 mm corundum; recommended for yeast and fungi)
|
740813.50 |
NucleoSpin® Bead Tubes
Type D |
50 |
2 mL tubes with steel balls for sample homogenization
(3 mm steel balls; recommended for insects, crustaceans, lipid-rich tissue) |
740814.50 |
NucleoSpin® Bead Tubes
Type E |
50 |
2 mL tubes with steel balls and glass beads for sample homogenization
(3 mm steel balls and 40–400 μm glass beads; recommended for bacteria within insects or tissue samples) |
740815.50 |
|
