Features |
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NEW generation of plasmid midi preparation |
• New column design (snap-off column) for vacuum processing of large sample volumes • Transfection-grade plasmid DNA for sensitive downstream applications • Isolate up to 700 μg plasmid DNA in only 35 minutes
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Technology |
Silica-membrane technology |
Processing |
Vacuum processing (e.g., using NucleoVac 24 Vacuum Manifold), centrifugation for elution
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Format |
Snap-off column |
Endotoxin level |
< 50 EU/µg DNA (EU = Endotoxin Units) |
Sample material |
Up to 50 mL E. coli culture (OD600=5) |
Lysate clarification |
NucleoSpin® Plasmid Filter Columns (2 min centrifugation) |
Vector size |
≤ 25 kbp |
Typical yield |
400–700 µg (50 mL culture, OD600=4, high-copy plasmid) |
A260/A280 |
1.8–1.9 |
Elution volume |
200–500 µL |
Preparation time |
35 min/6 preps |
Binding capacity |
1.5 mg |
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Applications |
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• Typical downstream applications: transfection of common cell lines (lipofection, electroporation) • Also suitable for standard molecular biology application such as cloning and sequencing
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Ordering information
Product |
Preps
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Specification |
REF |
NucleoSnap® Plasmid Midi |
10
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NucleoSnap® Plasmid Columns, NucleoSpin Plasmid Filter Columns, buffers, RNase A
|
740494.10 |
|
50
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as above |
740494.50 |
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Product accessories |
Pack of
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Specification |
REF |
NucleoVac 24 Vacuum Manifold |
1
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Vacuum manifold for processing NucleoSnap®or NucleoSpin® columns |
740299 |
NucleoVac Mini Adapter |
100
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Disposable adapters for processing NucleoSpin® or NucleoSnap® columns on the NucleoVac 24 Vacuum Manifold |
740297.100 |
NucleoVac Stop-cock |
24
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Stop-cocks for processing samples with different flow rates on the NucleoVac 24 Vacuum Manifold |
740298.24 |
Vacuum Regulator |
1
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For controlling of vacuum |
740641 |
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