| Features |
| |
| NEW generation of plasmid midi preparation |
|
• New column design (snap-off column) for vacuum processing
of large sample volumes
• Transfection-grade plasmid DNA for sensitive downstream applications
• Isolate up to 700 μg plasmid DNA in only 35 minutes
|
| |
|
| Technology |
Silica-membrane technology |
| Processing |
Vacuum processing (e.g., using NucleoVac 24 Vacuum Manifold),
centrifugation for elution
|
| Format |
Snap-off column |
| Endotoxin level |
< 50 EU/µg DNA (EU = Endotoxin Units) |
| Sample material |
Up to 50 mL E. coli culture (OD600=5) |
| Lysate clarification |
NucleoSpin® Plasmid Filter Columns (2 min centrifugation) |
| Vector size |
≤ 25 kbp |
| Typical yield |
400–700 µg (50 mL culture, OD600=4, high-copy plasmid) |
| A260/A280 |
1.8–1.9 |
| Elution volume |
200–500 µL |
| Preparation time |
35 min/6 preps |
| Binding capacity |
1.5 mg |
| |
| Applications |
| |
|
• Typical downstream applications: transfection of common cell lines (lipofection, electroporation)
• Also suitable for standard molecular biology application such as cloning and sequencing
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|
| |
|
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Ordering information
| Product |
Preps
|
Specification |
REF |
| NucleoSnap® Plasmid Midi |
10
|
NucleoSnap® Plasmid Columns, NucleoSpin Plasmid Filter Columns, buffers, RNase A
|
740494.10 |
| |
50
|
as above |
740494.50 |
| |
|
|
|
| Product accessories |
Pack of
|
Specification |
REF |
| NucleoVac 24 Vacuum Manifold |
1
|
Vacuum manifold for processing NucleoSnap®or NucleoSpin® columns |
740299 |
| NucleoVac Mini Adapter |
100
|
Disposable adapters for processing NucleoSpin® or NucleoSnap® columns on the NucleoVac 24 Vacuum Manifold |
740297.100 |
| NucleoVac Stop-cock |
24
|
Stop-cocks for processing samples with different flow rates on the NucleoVac 24 Vacuum Manifold |
740298.24 |
| Vacuum Regulator |
1
|
For controlling of vacuum |
740641 |
|
