Features |
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PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume |

• Two applications in one kit – one buffer with optimal performance for both applications • High recoveries for fragments down to 50 bp • Minimized elution volume of 15 μL – highly concentrated DNA • Binding buffer with pH indicator • Separate buffers for single stranded DNA or SDS containing samples available • Suitable for all gel buffer systems (e.g., TAE, TBE)
• Also available for QIAcube® (please see "Ordering information" and contact Technical Service at techbio@mn-net.com for more information).
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Technology |
Silica-membrane technology |
Format |
Mini spin columns |
Sample material |
< 400 µL PCR reaction mixture < 400 mg TAE / TBE agarose gel
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Fragment size |
50 bp–approx. 20 kbp |
Typical recovery |
70–95% |
A260/A280 |
1.8–1.9 |
Elution volume |
15–30 µL |
Preparation time |
10 min/6 preps |
Binding capacity |
25 µg |
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Applications |
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• Purification of PCR products • Extraction of DNA from agarose gels • DNA extraction from polyacrylamide gels • Clean-up of single stranded DNA and RNA extraction from agarose gels (Binding Buffer NTC required, not included in the kit, see “Ordering information”) • Clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the kit, see “Ordering information”) • Typical downstream applications: cloning, sequencing, PCR, restriction analysis
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• NucleoSpin® Gel and PCR Clean-up ensures complete removal of contaminations such as: - nucleotides, primers - enzymes - mineral oil - PCR additives (e.g., salts, betaine, DMSO) - detergents (e.g., Tween 20, Triton X-100) - dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti®-Safe GelStain, DNA SafeStain) |
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Ordering information
Product |
Preps
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Specification |
REF |
NucleoSpin® Gel and PCR Clean-up |
10
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NucleoSpin® Gel and PCR Clean-up Columns, Collection Tubes (2 mL), buffers |
740609.10 |
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50
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as above |
740609.50 |
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250
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as above |
740609.250 |
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240
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Kit for use on QIAcube®* NucleoSpin® Gel and PCR Clean-up Columns, buffers |
740609.240C |
Product accessories |
Pack of
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Specification |
REF |
Buffer NTB |
150 mL
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for clean-up of SDS-containing samples |
740595.150 |
Buffer NTC |
100 mL
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for clean-up of single stranded DNA |
740654.100 |
* NucleoSpin® kits on QIAcube®: The usage of NucleoSpin® kits on the QIAcube® is at your own liability. MACHEREY-NAGEL is not responsible for loss of warranty claims or other consequences. |
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