| Features |
| |
| PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume |
|
• Two applications in one kit – one buffer with optimal performance for both applications
• High recoveries for fragments down to 50 bp
• Minimized elution volume of 15 μL – highly concentrated DNA
• Binding buffer with pH indicator
• Separate buffers for single stranded DNA or SDS containing samples available
• Suitable for all gel buffer systems (e.g., TAE, TBE)
• Also available for QIAcube® (please see "Ordering information" and contact Technical Service
at techbio@mn-net.com for more information).
|
| |
|
| Technology |
Silica-membrane technology |
| Format |
Mini spin columns |
| Sample material |
< 400 µL PCR reaction mixture
< 400 mg TAE / TBE agarose gel
|
| Fragment size |
50 bp–approx. 20 kbp |
| Typical recovery |
70–95% |
| A260/A280 |
1.8–1.9 |
| Elution volume |
15–30 µL |
| Preparation time |
10 min/6 preps |
| Binding capacity |
25 µg |
| |
| Applications |
| |
|
• Purification of PCR products
• Extraction of DNA from agarose gels
• DNA extraction from polyacrylamide gels
• Clean-up of single stranded DNA and RNA extraction from agarose gels (Binding Buffer NTC required, not included in the kit, see “Ordering information”)
• Clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the
kit, see “Ordering information”)
• Typical downstream applications: cloning, sequencing, PCR, restriction analysis
|
• NucleoSpin® Gel and PCR Clean-up ensures complete removal of contaminations such as:
- nucleotides, primers
- enzymes
- mineral oil
- PCR additives (e.g., salts, betaine, DMSO)
- detergents (e.g., Tween 20, Triton X-100)
- dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti®-Safe GelStain, DNA SafeStain) |
|
| |
|
|
Ordering information
| Product |
Preps
|
Specification |
REF |
| NucleoSpin® Gel and PCR Clean-up |
10
|
NucleoSpin® Gel and PCR Clean-up Columns,
Collection Tubes (2 mL), buffers |
740609.10 |
| |
50
|
as above |
740609.50 |
| |
250
|
as above |
740609.250 |
| |
|
|
|
| |
240
|
Kit for use on QIAcube®*
NucleoSpin® Gel and PCR Clean-up Columns, buffers |
740609.240C |
| Product accessories |
Pack of
|
Specification |
REF |
| Buffer NTB |
150 mL
|
for clean-up of SDS-containing samples |
740595.150 |
| Buffer NTC |
100 mL
|
for clean-up of single stranded DNA |
740654.100 |
* NucleoSpin® kits on QIAcube®: The usage of NucleoSpin® kits on the QIAcube® is at your own liability.
MACHEREY-NAGEL is not responsible for loss of warranty claims or other consequences. |
|
