Features |
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The second generation for rapid isolation of genomic DNA from plant samples with higher yield and quality |
• Two alternative lysis buffers included for optimal processing of various samples and highest flexibility: Lysis Buffer PL1, based on CTAB lysis method Lysis Buffer PL2, based on SDS lysis method • NucleoSpin® Filters included for clarification of lysate • Improved buffer compositions – higher yield and purity • Optimized silica membrane – improved DNA binding • RNase A included |
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Technology |
Silica-membrane technology |
Format |
Mini spin columns |
Sample material |
< 100 mg plant tissue (wet weight), < 20 mg plant tissue (dry weight)
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Lysate clarification |
NucleoSpin® Filters |
Fragment size |
50 bp–approx. 50 kbp |
Typical yield |
1–30 µg (100 mg plant tissue, wet weight) |
A260/A280 |
1.8–1.9 |
Elution volume |
50–100 µL |
Preparation time |
30 min/prep |
Binding capacity |
50 µg |
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Applications |
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• DNA from plant cells and tissue • DNA from fungi • Typical downstream applications: PCR, Southern blotting, enzymatic reactions |
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Ordering information
Product |
Preps
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Specification |
REF |
NucleoSpin® Plant II |
10
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NucleoSpin® Plant II Columns, NucleoSpin Filters, Collection Tubes (2 mL), buffers, RNase A |
740770.10 |
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50
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as above |
740770.50 |
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250
|
as above |
740770.250 |
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