| Features |
| |
The second generation for rapid isolation of genomic DNA from plant samples
with higher yield and quality |
• Two alternative lysis buffers included for optimal processing of various samples
and highest flexibility:
Lysis Buffer PL1, based on CTAB lysis method
Lysis Buffer PL2, based on SDS lysis method
• NucleoSpin® Filters included for clarification of lysate
• Improved buffer compositions – higher yield and purity
• Optimized silica membrane – improved DNA binding
• RNase A included |
| |
|
| Technology |
Silica-membrane technology |
| Format |
Mini spin columns |
| Sample material |
< 100 mg plant tissue (wet weight), < 20 mg plant tissue (dry weight)
|
| Lysate clarification |
NucleoSpin® Filters |
| Fragment size |
50 bp–approx. 50 kbp |
| Typical yield |
1–30 µg (100 mg plant tissue, wet weight) |
| A260/A280 |
1.8–1.9 |
| Elution volume |
50–100 µL |
| Preparation time |
30 min/prep |
| Binding capacity |
50 µg |
| |
| Applications |
| |
• DNA from plant cells and tissue
• DNA from fungi
• Typical downstream applications: PCR, Southern blotting, enzymatic reactions |
|
| |
|
|
Ordering information
| Product |
Preps
|
Specification |
REF |
| NucleoSpin® Plant II |
10
|
NucleoSpin® Plant II Columns, NucleoSpin Filters, Collection Tubes (2 mL), buffers, RNase A |
740770.10 |
| |
50
|
as above |
740770.50 |
| |
250
|
as above |
740770.250 |
|
