NUCLEOSPIN RNA

Código: 740955 - Referência: NUCLEOSPIN RNA

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Features 
 
Mini spin kit for the isolation of RNA of highest integrity

• rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
• NucleoSpin® Filters (shredders) – efficient sample homogenization and reduction of viscosity
• Up to 70 μg ready-to-use RNA
• Parallel purification of genomic DNA by using the NucleoSpin® RNA/DNA Buffer Set

• Also available for QIAcube® (please see "Ordering information" and contact Technical Service
   at 
techbio@mn-net.com for more information).

   
Technology Silica-membrane technology
Format Mini spin columns
Sample material

< 5 x 10cultured cells, 
< 109 bacterial cells, 
< 108 yeast cells, 
< 30 mg human / animal tissue

Fragment size > 200 nt
Typical yield

14 μg (106 HeLa cells), 70 μg (109 bacterial cells)

A260/A280 1.9–2.1
Typical RIN (RNA integrity number) > 9
Elution volume 40–120 µL
Preparation time 35 min/6 preps
Binding capacity 200 µg
 

Applications*

 
• RNA isolation from cultured cells, tissue (standard protocol)
• RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
• RNA from ≤ 100 μL biological fluids
• RNA clean-up from reaction mixtures
• RNA from samples stored in RNAlater®
• RNA from saliva samples collected with Oragene®RNA (Genotek)
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, 
  array technology, RNase protection assays
 

* Kits to be used for research purposes only

  

 

     

Ordering information

Product

Preps

Specification REF
NucleoSpin® RNA

10

NucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase 740955.10
 

50

as above 740955.50
 

250

as above 740955.250
       
 

240

Kit for use on QIAcube®* 
NucleoSpin RNA® Columns with Collection Tubes, NucleoSpin® Filters, buffers, RNase-free rDNase
740955.240C
* NucleoSpin® kits on QIAcube®: The usage of NucleoSpin® kits on the QIAcube® is at your own liability. 
MACHEREY-NAGEL is not responsible for loss of warranty claims or other consequences.

 


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