| Features |
| |
| Improved DNA quality from formalin-fixed, paraffin-embedded samples – no xylene needed |
• Very easy paraffin removal – Paraffin Dissolver (patented technology) included
• Concentrated DNA by elution in small volume
• Overcoming formalin crosslinking of DNA by incubation in decrosslinking buffer
• High quality DNA for improved performance in PCR |
| |
|
| Technology |
Silica-membrane technology |
| Format |
Mini spin columns – XS design |
| Sample material |
≤ 7 sections (10 µm) of 250 mm2 total area (< 15 mg paraffin*)
|
| Typical yield |
Depending on amount and quality of the sample |
| DNA quality |
Strongly depending on sample quality |
| Elution volume |
5–30 µL |
| Preparation time |
70 min/6 preps (excl. lysis) |
| Binding capacity |
50 µg |
| |
| Applications** |
| |
• Rapid isolation of DNA from formalin-fixed, paraffin-embedded samples
• Isolation of DNA from fresh and archived FFPE samples
• Isolation of DNA from specimen of object slides
• Typical downstream application: PCR |
| |
* When using the standard protocol with Paraffin Dissolver. Larger quantities of paraffin can be processed when using additional Paraffin Dissolver or the protocol with xylene for deparaffinization.
** Kits to be used for research purposes only |
|
| |
|
|
Ordering information
| Product |
Preps
|
Specification |
REF |
| NucleoSpin® DNA FFPE XS |
10
|
NucleoSpin® DNA FFPE XS Columns, Collection Tubes (2 mL), Paraffin Dissolver, buffers, Proteinase K
|
740980.10 |
| |
50
|
as above |
740980.50 |
| |
250
|
as above |
740980.250 |
|
