NUCLEOSPIN RNA PLUS

Código: 740984 - Referência: NUCLEOSPIN RNA PLUS

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Features 
 
New generation for efficient and quick RNA isolation from cells and tissues

• NucleoSpin® gDNA Removal Column – no time consuming rDNase digestion
• Convenient handling – lysate clearing and gDNA removal with one column in one step
• New efficient lysis buffer – no b-mercaptoethanol or TCEP necessary

   
Technology Two column silica-membrane system
1. column for DNA removal
2. column for RNA isolation
Format Mini spin columns
Sample material

< 1 x 107 cultured cells, 
< 109 bacterial cells, 
< 108 yeast cells, 
< 30 mg human / animal tissue

Fragment size > 200 nt
Typical yield

HeLa cells 5 x 106: 40-60 µg
Mouse liver 20 mg: 80-100 µg
Mouse kidney 20 mg: 40-70 µg
Mouse spleen 5 mg: 30-60 µg

A260/A280 1.9–2.1
A260/A230 1.8-2.5
Typical RIN (RNA integrity number) > 9
Elution volume 30–120 µL
Preparation time 20 min/6 preps
Binding capacity 200 µg
 

Applications*

 
• RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
   RNase protection assays
 

* Kits to be used for research purposes only

  

 

     

Ordering information

Product

Preps

Specification REF
NucleoSpin® RNA Plus

10

NucleoSpin® RNA Plus Columns with Collection Tubes, NucleoSpin® gDNA Removal Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers 740984.10
 

50

as above 740984.50
 

250

as above 740984.250

 


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