Features |
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New generation for efficient and quick RNA isolation from cells and tissues |
• NucleoSpin® gDNA Removal Column – no time consuming rDNase digestion • Convenient handling – lysate clearing and gDNA removal with one column in one step • New efficient lysis buffer – no b-mercaptoethanol or TCEP necessary
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Technology |
Two column silica-membrane system 1. column for DNA removal 2. column for RNA isolation |
Format |
Mini spin columns |
Sample material |
< 1 x 107 cultured cells, < 109 bacterial cells, < 108 yeast cells, < 30 mg human / animal tissue
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Fragment size |
> 200 nt |
Typical yield |
HeLa cells 5 x 106: 40-60 µg Mouse liver 20 mg: 80-100 µg Mouse kidney 20 mg: 40-70 µg Mouse spleen 5 mg: 30-60 µg
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A260/A280 |
1.9–2.1 |
A260/A230 |
1.8-2.5 |
Typical RIN (RNA integrity number) |
> 9 |
Elution volume |
30–120 µL |
Preparation time |
20 min/6 preps |
Binding capacity |
200 µg |
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Applications*
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• RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays |
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* Kits to be used for research purposes only
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Ordering information
Product |
Preps
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Specification |
REF |
NucleoSpin® RNA Plus |
10
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NucleoSpin® RNA Plus Columns with Collection Tubes, NucleoSpin® gDNA Removal Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers |
740984.10 |
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50
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as above |
740984.50 |
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250
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as above |
740984.250 |
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