For detection and confirmation of coliform bacteria in water and foods

Composition

* Dehydrated powder, hygroscopic in nature, store in a dry place, in tightly-sealed containers below 25°C and protect from direct Sunlight.

Instructions for Use

Dissolve 40gms in 1000ml distilled water. Gently heat to boiling with gentle swirling and dissolve the medium completely. Sterilize by autoclaving at 15 psi (121 ºC) for 15 minutes and dispense required amounts in tubes containing inverted Durham’s tubes.

Appearance: Emerald green colour, clear solution without any precipitate

pH (at 25°C): 7.2 ± 0.2

Principle

BRILLIANT GREEN BILE BROTH 2% (BRILLIANT GREEN LACTOSE BILE BROTH 2%) is used for

the detection of coliform organisms in foods, dairy products, water and wastewater, as well as in other materials of sanitary importance. Brilliant Green Bile Broth 2% contains two inhibitors of both gram- positive and selected gram-negative organisms; i.e., Oxgall and Brilliant green dye. Peptic digest of animal tissue provides the essential nutrients for growth: nitrogen, vitamins, minerals and amino acids. Organisms, primarily coliforms, which are resistant to the action of the inhibitors and which ferment the Lactose, are able to replicate in this medium. Fermentation is detected by gas production. Gas production within 48 ± 3 hours is considered positive evidence of fermentation by coliform bacilli.

Interpretation

Cultural characteristics observed after inoculating (103 – 105CFU/ml), on incubation at 35ºC for 48 hours.

References

1. Wehr and Frank (ed.).Standard methods for the examination of dairy products, 17th ed. American Public Health Association, Washington, D.C. (2004).
2. U.S. Food and Drug Administration. Bacteriological analytical manual, online. AOAC International, Gaithersburg, Md. (2001).
3. Clesceri, Greenberg and Eaton (ed.). Standard methods for the examination of water and wastewater, 20th ed. American Public Health Association, Washington, D.C. (1998).
4. U.S. Food and Drug Administration. Bacteriological analytical manual, 8th ed. AOAC International, Gaithersburg, Md. (1995).