NUCLEOSPIN DNA STOOL

Categoria:
Features
Isolation of genomic DNA from stool samples

        

• Suitable for a any stool sample – high quality DNA from stool from carnivores,
omnivores, and herbivores
• NucleoSpin® Bead Tubes for efficient lysis included – compatible with the most
common disruption devices
• NucleoSpin® Inhibitor Removal Columns ensure highest purity of the isolated DNA

TechnologySilica-membrane technology combined with NucleoSpin® Bead Tubes Type A
FormatMini spin columns
Sample materialApprox. 200 mg (180–220 mg)* stool samples (fresh or frozen)
Fragment size200 bp–approx. 50 kbp
Typical yieldDepends on sample type, quality, and water content
A260/A2801.7–1.9
Elution volume30–100 µL
Preparation time60 min/10 preps
Binding capacity50 µg
* For human stool samples approx. 200 mg should be used. For animal stool samples – depending on the
species – a lower amount of sample material may be required for optimal results.
Applications
• Genomic DNA from bacterial and epithelial cells from stool samples
• Typical downstream applications: PCR, real-time PCR, Southern blotting, microarray technology

 

 

 

Ordering information

Product

Preps

SpecificationREF
NucleoSpin® DNA Stool

10

NucleoSpin® DNA Stool Columns, NucleoSpin®Bead Tubes Type A, NucleoSpin® Inhibitor Removal Columns, Collection Tubes (2 mL), Collection Tubes (2 mL, lid), buffers740472.10

50

as above740472.50
250as above740472.250

Product accessories

Pieces

SpecificationREF
MN Bead Tube Holder1Rubber-foam adapter for processing NucleoSpin® Bead Tubes with Vortex-Genie® 2740469
NucleoSpin® Bead Tubes
Type A
502 mL tubes with ceramic beads for sample homogenization
(0.6–0.8 mm ceramic beads; recommended for stool, soil, and sediments)
740786.50
NucleoSpin® Bead Tubes
Type B
502 ml tubes with glass beads for sample homogenization
(40–400 μm glass beads; recommended for bacteria)
740812.50
NucleoSpin® Bead Tubes
Type C
502 mL tubes with corundum for sample homogenization
(1–3 mm corundum; recommended for yeast and fungi)
740813.50
NucleoSpin® Bead Tubes
Type D
502 mL tubes with steel balls for sample homogenization
(3 mm steel balls; recommended for insects, crustaceans, lipid-rich tissue)
740814.50
NucleoSpin® Bead Tubes
Type E
502 mL tubes with steel balls and glass beads for sample homogenization
(3 mm steel balls and 40–400 μm glass beads; recommended for bacteria within insects or tissue samples)
740815.50

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