| Features | | | PCR clean-up and gel extraction – the two-in-one kit with optimized recovery and elution volume |  • Two applications in one kit – one buffer with optimal performance for both applications
• High recoveries for fragments down to 50 bp
• Minimized elution volume of 15 μL – highly concentrated DNA
• Binding buffer with pH indicator
• Separate buffers for single stranded DNA or SDS containing samples available
• Suitable for all gel buffer systems (e.g., TAE, TBE) • Also available for QIAcube® (please see “Ordering information” and contact Technical Service
at techbio@mn-net.com for more information). |
| | | Technology | Silica-membrane technology | | Format | Mini spin columns | | Sample material | < 400 µL PCR reaction mixture
< 400 mg TAE / TBE agarose gel | | Fragment size | 50 bp–approx. 20 kbp | | Typical recovery | 70–95% | | A260/A280 | 1.8–1.9 | | Elution volume | 15–30 µL | | Preparation time | 10 min/6 preps | | Binding capacity | 25 µg | | | Applications | | • Purification of PCR products
• Extraction of DNA from agarose gels
• DNA extraction from polyacrylamide gels
• Clean-up of single stranded DNA and RNA extraction from agarose gels (Binding Buffer NTC required, not included in the kit, see “Ordering information”)
• Clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the
kit, see “Ordering information”)
• Typical downstream applications: cloning, sequencing, PCR, restriction analysis | • NucleoSpin® Gel and PCR Clean-up ensures complete removal of contaminations such as:
– nucleotides, primers
– enzymes
– mineral oil
– PCR additives (e.g., salts, betaine, DMSO)
– detergents (e.g., Tween 20, Triton X-100)
– dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti®-Safe GelStain, DNA SafeStain) |
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