| Features | | The second generation for rapid isolation of genomic DNA from plant samples
with higher yield and quality | • Two alternative lysis buffers included for optimal processing of various samples
and highest flexibility:
Lysis Buffer PL1, based on CTAB lysis method
Lysis Buffer PL2, based on SDS lysis method
• NucleoSpin® Filters included for clarification of lysate
• Improved buffer compositions – higher yield and purity
• Optimized silica membrane – improved DNA binding
• RNase A included |
| | | Technology | Silica-membrane technology | | Format | Mini spin columns | | Sample material | < 100 mg plant tissue (wet weight), < 20 mg plant tissue (dry weight) | | Lysate clarification | NucleoSpin® Filters | | Fragment size | 50 bp–approx. 50 kbp | | Typical yield | 1–30 µg (100 mg plant tissue, wet weight) | | A260/A280 | 1.8–1.9 | | Elution volume | 50–100 µL | | Preparation time | 30 min/prep | | Binding capacity | 50 µg | | | Applications | | • DNA from plant cells and tissue
• DNA from fungi
• Typical downstream applications: PCR, Southern blotting, enzymatic reactions |
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