• Two alternative lysis buffers and a special additive (Enhancer SX) for optimal
processing of various soil samples – high yield and high purity
• NucleoSpin® Bead Tubes with ceramic beads for most efficient cell disruption
of Gram-positive and Gram-negative bacteria, archaea, yeast, fungi, and algae in soil,
sludge,or sediment
• NucleoSpin® Inhibitor Removal Column to eliminate all PCR inhibitors,
such as humic substances – DNA is ready-to-use for PCR without dilution
Technology
Silica-membrane technology
Format
Mini spin columns
Sample material
< 500 mg soil, sludge, or sediment
Typical yield
2–10 µg (500 mg soil)
Elution volume
30–100 µL
Preparation time
90 min/10 preps
Binding capacity
50 µg
Applications
• Total DNA from microorganisms in soil and sediment
• Quantify and detect microorganisms from environmental samples
• Typical downstream applications: PCR, real-time PCR, Southern blotting, microarray technology
References
PLoS One 2013 July;8(7):e67699. doi:101371/journal.pone.0067699. Comparison of DNA Extraction Methods in Analysis of Salivary Bacterial Communities
Lazarevic V, Gaia N, Girard M, Francois P, Schrenzel J.
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