CAMPYLOBACTER SUPPLEMENT-I (BLASER-WANG)

Description

Supplement contains antibiotics which are targetted to selective species in for isolation, cultivation and detection of haemolytic activity of Streptococci, Pneumococci and other fastidious microorganisms in these culture media’s Columbia Blood Agar Base (TM 071), Blood Agar Base No. 2 (TM 041), and Brucella Agar Base (TM 514).

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 Composition

Ingredients

Cephalothin                                        7.5mg

Vancomycin                                       5.0mg

Trimethoprim                                    2.5mg

Amphotericin B                                 1.00mg

Polymyxin B sulphate                     1250.0 IU

(per vial, sufficient for 500ml of sterilized medium)

 

  Storage

Vials should be stored in sealed container in cool, dry location and protected from direct sunlight.

 

  Instructions for Use

Aseptically rehydrate 1 vial contents into 2ml of sterile distilled water. Mix well and add into sterilized molten medium when cooled to 45 – 500C. Use the medium according to the manufacturer’s recommendation. Store the prepared media plates inverted at 4-8°C for not more than 7 days.

 

  Microbiological Tests

For TM 071 COLUMBIA BLOOD AGAR BASE and for TM 041 BLOOD AGAR BASE No. 2 – Cultural

characteristics observed after inoculating (103CFU/ml), on incubation at 35-37°C for 18- 48 hour.

 

Microorganisms

ATCC

Inoculum

(CFU/ml)

Growth

Growth with 5% of Sheep Blood

Hemolysis

Staphylococcus aureus

25923

103

Good

Good

Beta

Proteus miriabilis

12453

103

Inhibited

Inhibited

Streptococcus pneumoniae

6305

103

Good

Good

Alpha

Streptococcus pyogenes

19615

103

Good

Good

Beta

 

For TM 682 BRUCELLA AGAR BASE – Cultural characteristics observed after inoculating (103CFU/ml), on incubation at 35 ± 2 °C under 10% CO2 for 18 – 72 hours.

 

Microorganisms

ATCC

Inoculum

(CFU/ml)

Growth

Brucella melitensis

4309

103

Luxuriant

Brucella suis

4314

103

Luxuriant

Escherichia coli

25922

103

Inhibited

Staphylococcus aureus

25923

103

Inhibited

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