Kit de Extração

NUCLEOBOND XTRA MIDI

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Column filters		Column filters
Sample material	< 200 mL (high copy) < 400 mL (low copy)		< 600 mL (high copy) < 1200 mL (low copy)
Vector size	< 300 kbp		< 300 kbp
Typical yield	400 µg		1000 µg
A260/A280	1.80–1.95		1.80–1.95
Isopropanol precipitation	Xtra Midi Centrifugation	Xtra Midi Plus NucleoBond®Finalizer	Xtra Maxi Centrifugation	Xtra Maxi Plus NucleoBond®Finalizer Large
Preparation time	70 min/prep	30 min/prep	75 min/prep	35 min/prep

Applications

• High-copy plasmids from E. coli • Low-copy plasmids from E. coli (increased buffer volumes required, see “Ordering information”)

Ordering information

Product	Preps	Specification	REF
NucleoBond® Xtra Midi kits
NucleoBond® Xtra Midi	10	NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A	740410.10
	50	as above	740410.50
	100	as above	740410.100
NucleoBond® Xtra Midi Plus	10	NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizers, buffers, RNase A	740412.10
	50	as above	740412.50
NucleoBond® Xtra Maxi kits
NucleoBond® Xtra Maxi	10	NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A	740414.10
	50	as above	740414.50
	100	as above	740414.100
NucleoBond® Xtra Maxi Plus	10	NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizers Large, buffers, RNase A	740416.10
	50	as above	740416.50

Product accessories	Pack of	Specification	REF
NucleoBond® Xtra Combi Rack	1	for use with NucleoBond® Xtra Midi, Maxi, BAC, AX 100, AX 500, AX 2000, and AX 10000, BAC 100 Columns	740415
NucleoBond® Smart Rack	1	for use with NucleoBond® Xtra Midi, Xtra Maxi,Xtra BAC, AX 20, AX 100,AX 500, AX 2000, and AX 10000,	740413
NucleoBond® Xtra Buffer Set I	1	for isolation of low-copy plasmids, cosmids, BACs, PACs, and P1 constructs, only applicable with NucleoBond® Xtra kits, sufficient for 12 Xtra Maxi preps or 18 Xtra Midi preps. Set includes 150 mL buffers RES, LYS, NEU, and RNase A	740417

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