NUCLEOBOND XTRA MIDI

Categoria:
Column filters Column filters
Sample material < 200 mL (high copy)
< 400 mL (low copy)
< 600 mL (high copy)
< 1200 mL (low copy)

Vector size

< 300 kbp < 300 kbp
Typical yield 400 µg 1000 µg
A260/A280 1.80–1.95 1.80–1.95
Isopropanol precipitation

Xtra Midi

Centrifugation

Xtra Midi Plus

NucleoBond®Finalizer

Xtra Maxi

Centrifugation

Xtra Maxi Plus

NucleoBond®Finalizer Large

Preparation time 70 min/prep 30 min/prep 75 min/prep 35 min/prep
Applications
• High-copy plasmids from E. coli
• Low-copy plasmids from E. coli (increased buffer volumes required, see “Ordering information”)

 

 

 

Ordering information

Product

Preps

Specification REF
NucleoBond® Xtra Midi kits
NucleoBond® Xtra Midi

10

NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A 740410.10

50

as above 740410.50

100

as above 740410.100
NucleoBond® Xtra Midi Plus

10

NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizers, buffers, RNase A

740412.10

50

as above 740412.50
NucleoBond® Xtra Maxi kits
NucleoBond® Xtra Maxi

10

NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A 740414.10

50

as above 740414.50

100

as above 740414.100
NucleoBond® Xtra Maxi Plus

10

NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizers Large, buffers, RNase A

740416.10

50

as above 740416.50
Product accessories

Pack of

Specification REF
NucleoBond® Xtra Combi Rack

1

for use with NucleoBond® Xtra Midi, Maxi, BAC, AX 100, AX 500, AX 2000, and AX 10000, BAC 100 Columns 740415
NucleoBond® Smart Rack

1

for use with NucleoBond® Xtra Midi, Xtra Maxi,Xtra BAC, AX 20, AX 100,AX 500, AX 2000, and AX 10000, 740413
NucleoBond® Xtra Buffer Set I

1

for isolation of low-copy plasmids, cosmids, BACs, PACs, and P1 constructs,
only applicable with NucleoBond® Xtra kits, sufficient for 12 Xtra Maxi preps or 18 Xtra Midi preps. Set includes 150 mL buffers RES, LYS, NEU, and RNase A
740417

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