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NUCLEOSNAP PLASMID MIDI COLUMNS

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Features
NEW generation of plasmid midi preparation

     
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• New column design (snap-off column) for vacuum processing
of large sample volumes
• Transfection-grade plasmid DNA for sensitive downstream applications
• Isolate up to 700 μg plasmid DNA in only 35 minutes
Technology Silica-membrane technology
Processing Vacuum processing (e.g., using NucleoVac 24 Vacuum Manifold),
centrifugation for elution
Format Snap-off column
Endotoxin level < 50 EU/µg DNA (EU = Endotoxin Units)
Sample material Up to 50 mL E. coli culture (OD600=5)
Lysate clarification NucleoSpin® Plasmid Filter Columns (2 min centrifugation)
Vector size ≤ 25 kbp
Typical yield 400–700 µg (50 mL culture, OD600=4, high-copy plasmid)
A260/A280 1.8–1.9
Elution volume 200–500 µL
Preparation time 35 min/6 preps
Binding capacity 1.5 mg
Applications
• Typical downstream applications: transfection of common cell lines (lipofection, electroporation)
• Also suitable for standard molecular biology application such as cloning and sequencing

 

 

 Ordering information

Product

Preps

 Specification REF
NucleoSnap® Plasmid Midi

10

NucleoSnap® Plasmid Columns, NucleoSpin Plasmid Filter Columns, buffers, RNase A

 740494.10

50

 as above 740494.50
Product accessories

Pack of

 Specification REF
NucleoVac 24 Vacuum Manifold

1

 Vacuum manifold for processing NucleoSnap®or NucleoSpin® columns 740299
NucleoVac Mini Adapter

100

 Disposable adapters for processing NucleoSpin® or NucleoSnap® columns on the NucleoVac 24 Vacuum Manifold 740297.100
NucleoVac Stop-cock

24

 Stop-cocks for processing samples with different flow rates on the NucleoVac 24 Vacuum Manifold 740298.24
Vacuum Regulator

1

 For controlling of vacuum 740641

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