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NUCLEOSNAP PLASMID MIDI COLUMNS

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Features
NEW generation of plasmid midi preparation

     
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• New column design (snap-off column) for vacuum processing
of large sample volumes
• Transfection-grade plasmid DNA for sensitive downstream applications
• Isolate up to 700 μg plasmid DNA in only 35 minutes

TechnologySilica-membrane technology
ProcessingVacuum processing (e.g., using NucleoVac 24 Vacuum Manifold),
centrifugation for elution
FormatSnap-off column
Endotoxin level< 50 EU/µg DNA (EU = Endotoxin Units)
Sample materialUp to 50 mL E. coli culture (OD600=5)
Lysate clarificationNucleoSpin® Plasmid Filter Columns (2 min centrifugation)
Vector size≤ 25 kbp
Typical yield400–700 µg (50 mL culture, OD600=4, high-copy plasmid)
A260/A2801.8–1.9
Elution volume200–500 µL
Preparation time35 min/6 preps
Binding capacity1.5 mg
Applications
• Typical downstream applications: transfection of common cell lines (lipofection, electroporation)
• Also suitable for standard molecular biology application such as cloning and sequencing

 

 

 Ordering information

Product

Preps

SpecificationREF
NucleoSnap® Plasmid Midi

10

NucleoSnap® Plasmid Columns, NucleoSpin Plasmid Filter Columns, buffers, RNase A

740494.10

50

as above740494.50
Product accessories

Pack of

SpecificationREF
NucleoVac 24 Vacuum Manifold

1

Vacuum manifold for processing NucleoSnap®or NucleoSpin® columns740299
NucleoVac Mini Adapter

100

Disposable adapters for processing NucleoSpin® or NucleoSnap® columns on the NucleoVac 24 Vacuum Manifold740297.100
NucleoVac Stop-cock

24

Stop-cocks for processing samples with different flow rates on the NucleoVac 24 Vacuum Manifold740298.24
Vacuum Regulator

1

For controlling of vacuum740641

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