NUCLEOSNAP PLASMID MIDI COLUMNS

Features

NEW generation of plasmid midi preparation


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• New column design (snap-off column) for vacuum processing
of large sample volumes
• Transfection-grade plasmid DNA for sensitive downstream applications
• Isolate up to 700 μg plasmid DNA in only 35 minutes


Technology	Silica-membrane technology
Processing	Vacuum processing (e.g., using NucleoVac 24 Vacuum Manifold), centrifugation for elution
Format	Snap-off column
Endotoxin level	< 50 EU/µg DNA (EU = Endotoxin Units)
Sample material	Up to 50 mL E. coli culture (OD600=5)
Lysate clarification	NucleoSpin® Plasmid Filter Columns (2 min centrifugation)
Vector size	≤ 25 kbp
Typical yield	400–700 µg (50 mL culture, OD600=4, high-copy plasmid)
A260/A280	1.8–1.9
Elution volume	200–500 µL
Preparation time	35 min/6 preps
Binding capacity	1.5 mg

Applications

• Typical downstream applications: transfection of common cell lines (lipofection, electroporation) • Also suitable for standard molecular biology application such as cloning and sequencing

Ordering information

Product	Preps	Specification	REF
NucleoSnap® Plasmid Midi	10	NucleoSnap® Plasmid Columns, NucleoSpin Plasmid Filter Columns, buffers, RNase A	740494.10
	50	as above	740494.50

Product accessories	Pack of	Specification	REF
NucleoVac 24 Vacuum Manifold	1	Vacuum manifold for processing NucleoSnap®or NucleoSpin® columns	740299
NucleoVac Mini Adapter	100	Disposable adapters for processing NucleoSpin® or NucleoSnap® columns on the NucleoVac 24 Vacuum Manifold	740297.100
NucleoVac Stop-cock	24	Stop-cocks for processing samples with different flow rates on the NucleoVac 24 Vacuum Manifold	740298.24
Vacuum Regulator	1	For controlling of vacuum	740641

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