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| Technology |
Silica-membrane technology |
| Processing |
Vacuum processing (e.g., using NucleoVac 24 Vacuum Manifold),
centrifugation for elution |
| Format |
Snap-off column |
| Endotoxin level |
< 50 EU/µg DNA (EU = Endotoxin Units) |
| Sample material |
Up to 50 mL E. coli culture (OD600=5) |
| Lysate clarification |
NucleoSpin® Plasmid Filter Columns (2 min centrifugation) |
| Vector size |
≤ 25 kbp |
| Typical yield |
400–700 µg (50 mL culture, OD600=4, high-copy plasmid) |
| A260/A280 |
1.8–1.9 |
| Elution volume |
200–500 µL |
| Preparation time |
35 min/6 preps |
| Binding capacity |
1.5 mg |
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| Applications |
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• Typical downstream applications: transfection of common cell lines (lipofection, electroporation)
• Also suitable for standard molecular biology application such as cloning and sequencing |
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