NUCLEOSPIN DNA LIPID TISSUE

Categoria:
Features 

Isolation of genomic DNA from lipid-rich tissue

        

• Special buffer composition for efficient removal of lipids
• NucleoSpin® Bead Tubes for efficient lysis included – compatible with the
most common disruption devices
• Fast and convenient procedure without RNA contamination

Technology Silica-membrane technology combined with NucleoSpin® Bead Tubes
Type D
Format Mini spin columns
Sample material < 40 mg fresh or frozen, lipid-rich tissue
(e.g., brain, adipose tissue, fatty fish tissue)
Fragment size 200 bp–approx. 50 kbp
Typical yield Depends on sample type, quality, and water content
A260/A280 1.7–1.9
Elution volume 25–200 µL
Preparation time 35 min/6 preps
Binding capacity 60 µg
Applications*
• Genomic DNA from any type of tissue with a high lipid content
• Typical downstream applications: multiplex PCR, Southern blotting, enzymatic reactions
* Kits to be used for research purposes only

 

Ordering information 

Product

Preps

Specification REF
NucleoSpin® DNA Lipid Tissue

10

NucleoSpin® DNA Lipid Tissue Columns, NucleoSpin® Bead Tubes Type D, Collection Tubes (2 mL), buffers 740471.10

50

as above 740471.50

Product accessories

Pieces

Specification REF
MN Bead Tube Holder 1 Rubber-foam adapter for processing NucleoSpin® Bead Tubes with Vortex-Genie® 2 740469
NucleoSpin® Bead Tubes
Type A
50 2 mL tubes with ceramic beads for sample homogenization
(0.6–0.8 mm ceramic beads; recommended for stool, soil, and sediments)
740786.50
NucleoSpin® Bead Tubes
Type B
50 2 ml tubes with glass beads for sample homogenization
(40–400 μm glass beads; recommended for bacteria)
740812.50
NucleoSpin® Bead Tubes
Type C
50 2 mL tubes with corundum for sample homogenization
(1–3 mm corundum; recommended for yeast and fungi)
740813.50
NucleoSpin® Bead Tubes
Type D
50 2 mL tubes with steel balls for sample homogenization
(3 mm steel balls; recommended for insects, crustaceans, lipid-rich tissue)
740814.50
NucleoSpin® Bead Tubes
Type E
50 2 mL tubes with steel balls and glass beads for sample homogenization
(3 mm steel balls and 40–400 μm glass beads; recommended for bacteria within insects or tissue samples)
740815.50

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