NUCLEOSPIN MICROBIAL DNA

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Features 

Kit for purification of high-quality DNA from gram-positive and gram-negative bacteria, yeast, and fungi

• Suitable for a large variety of starting materials
• NucleoSpin® Bead Tubes for efficient lysis included – compatible with many disruption devices
• Fast and easy procedure – Liquid Proteinase K included, no additional enzyme required

 

Technology Silica-membrane technology
Format Mini spin columns
Sample material < 40 mg wet weight cell pellet
Fragment size 200 bp–approx. 50 kbp
Typical yield Depends on sample type and disruption
Approx. 5-25 µg (30 mg wet weight cell pellet)
A260/A280 1.6–2.0
Elution volume 100–200 µL
Preparation time 35 min/prep
Binding capacity 60 µg
Applications*
• Total DNA from microbial cultures
• Typical downstream applications: PCR, real-time PCR, southern blotting, enzymatic reactions
* Kits to be used for research purposes only

 

 

Ordering information 

Product

Preps

Specification REF
NucleoSpin® Microbial DNA

10

NucleoSpin® Microbial DNA Columns, NucleoSpin® Bead Tubes Type B, Collection
Tubes (2 mL), buffers, Liquid Proteinase K
740235.10

50

as above 740235.50

250

as above 740235.250
Product

Pieces

Specification REF
NucleoSpin® Bead Tubes
Type A
50 2 ml tubes with ceramic beads
(included in the NucleoSpin® Soil kit)
740786.50
NucleoSpin® Bead Tubes
Type B
50 2 ml tubes with glass beads
(included in the NucleoSpin® Microbial kit)
740812.50
NucleoSpin® Bead Tubes
Type C
50 2 mL tubes with corundum 7408

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