NUCLEOSPIN MICROBIAL DNA

Features

Kit for purification of high-quality DNA from gram-positive and gram-negative bacteria, yeast, and fungi

• Suitable for a large variety of starting materials
• NucleoSpin® Bead Tubes for efficient lysis included – compatible with many disruption devices
• Fast and easy procedure – Liquid Proteinase K included, no additional enzyme required


Technology	Silica-membrane technology
Format	Mini spin columns
Sample material	< 40 mg wet weight cell pellet
Fragment size	200 bp–approx. 50 kbp
Typical yield	Depends on sample type and disruption Approx. 5-25 µg (30 mg wet weight cell pellet)
A260/A280	1.6–2.0
Elution volume	100–200 µL
Preparation time	35 min/prep
Binding capacity	60 µg

Applications*

• Total DNA from microbial cultures • Typical downstream applications: PCR, real-time PCR, southern blotting, enzymatic reactions

* Kits to be used for research purposes only

Ordering information

Product	Preps	Specification	REF
NucleoSpin® Microbial DNA	10	NucleoSpin® Microbial DNA Columns, NucleoSpin® Bead Tubes Type B, Collection Tubes (2 mL), buffers, Liquid Proteinase K	740235.10
	50	as above	740235.50
	250	as above	740235.250

Product	Pieces	Specification	REF

NucleoSpin® Bead Tubes Type A	50	2 ml tubes with ceramic beads (included in the NucleoSpin® Soil kit)	740786.50
NucleoSpin® Bead Tubes Type B	50	2 ml tubes with glass beads (included in the NucleoSpin® Microbial kit)	740812.50
NucleoSpin® Bead Tubes Type C	50	2 mL tubes with corundum	7408

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