| Features |
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| Isolation of RNA from fresh or frozen whole blood (human or animal) |
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• Direct total blood lysis – very simple and convenient procedure (patent pending)
• No selective erythrocyte lysis at 4 °C – complete processing at room temperature
• Superior RNA yield and quality from up to 400 µL whole blood
• Efficient on-column DNA removal for an increased sensitivity in downstream applications
• Compatible with common blood collection tubes and anticoagulants
(e.g., EDTA, citrate, and heparin) |
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| Technology |
Silica-membrane technology |
| Format |
Mini spin columns |
| Sample material |
< 400 µL whole blood (fresh or frozen) |
| Fragment size |
> 200 nt |
| Typical yield |
1–8 µg (400 µL whole blood)* |
| Typical purity (A260/A280) |
1.9–2.1 |
| Elution volume |
40–120 µL |
| Preparation time |
55 min/6 preps |
| Binding capacity |
200 µg |
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| * RNA yield strongly depends on the leukocyte number in each individual blood sample. |
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Applications*
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• Isolation of RNA from fresh or frozen whole blood from common blood collection tubes
(EDTA, citrate, heparin)
• Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology |
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| * Kits to be used for research purposes only |
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