| Features | | | Isolation of RNA from fresh or frozen whole blood (human or animal) | • Direct total blood lysis – very simple and convenient procedure (patent pending)
• No selective erythrocyte lysis at 4 °C – complete processing at room temperature
• Superior RNA yield and quality from up to 400 µL whole blood
• Efficient on-column DNA removal for an increased sensitivity in downstream applications
• Compatible with common blood collection tubes and anticoagulants
(e.g., EDTA, citrate, and heparin) |
| | | Technology | Silica-membrane technology | | Format | Mini spin columns | | Sample material | < 400 µL whole blood (fresh or frozen) | | Fragment size | > 200 nt | | Typical yield | 1–8 µg (400 µL whole blood)* | | Typical purity (A260/A280) | 1.9–2.1 | | Elution volume | 40–120 µL | | Preparation time | 55 min/6 preps | | Binding capacity | 200 µg | | | | * RNA yield strongly depends on the leukocyte number in each individual blood sample. | | Applications* | | • Isolation of RNA from fresh or frozen whole blood from common blood collection tubes
(EDTA, citrate, heparin)
• Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology | | | * Kits to be used for research purposes only |
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