NUCLEOSPIN RNA BLOOD

Categoria:
Features
Isolation of RNA from fresh or frozen whole blood (human or animal)

• Direct total blood lysis – very simple and convenient procedure (patent pending)
• No selective erythrocyte lysis at 4 °C – complete processing at room temperature
• Superior RNA yield and quality from up to 400 µL whole blood
• Efficient on-column DNA removal for an increased sensitivity in downstream applications
• Compatible with common blood collection tubes and anticoagulants
(e.g., EDTA, citrate, and heparin)

Technology Silica-membrane technology
Format Mini spin columns
Sample material < 400 µL whole blood (fresh or frozen)
Fragment size > 200 nt
Typical yield 1–8 µg (400 µL whole blood)*
Typical purity (A260/A280) 1.9–2.1
Elution volume 40–120 µL
Preparation time 55 min/6 preps
Binding capacity 200 µg
RNA yield strongly depends on the leukocyte number in each individual blood sample.

Applications*

• Isolation of RNA from fresh or frozen whole blood from common blood collection tubes
(EDTA, citrate, heparin)
• Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology
* Kits to be used for research purposes only

 

 

Ordering information

Product

Preps

Specification REF
NucleoSpin® RNA Blood

10

NucleoSpin® RNA Blood Columns with Collection Tubes, Collection Tubes (2 mL), buffers, RNase-free rDNase, Liquid Proteinase K 740200.10

50

as above 740200.50

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