Features |
|
Isolation of RNA from fresh or frozen whole blood (human or animal) |
• Direct total blood lysis – very simple and convenient procedure (patent pending)
• No selective erythrocyte lysis at 4 °C – complete processing at room temperature
• Superior RNA yield and quality from up to 400 µL whole blood
• Efficient on-column DNA removal for an increased sensitivity in downstream applications
• Compatible with common blood collection tubes and anticoagulants
(e.g., EDTA, citrate, and heparin) |
|
|
Technology |
Silica-membrane technology |
Format |
Mini spin columns |
Sample material |
< 400 µL whole blood (fresh or frozen) |
Fragment size |
> 200 nt |
Typical yield |
1–8 µg (400 µL whole blood)* |
Typical purity (A260/A280) |
1.9–2.1 |
Elution volume |
40–120 µL |
Preparation time |
55 min/6 preps |
Binding capacity |
200 µg |
|
|
* RNA yield strongly depends on the leukocyte number in each individual blood sample. |
|
Applications*
|
|
• Isolation of RNA from fresh or frozen whole blood from common blood collection tubes
(EDTA, citrate, heparin)
• Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology |
|
* Kits to be used for research purposes only |
|
Avaliações
Não há avaliações ainda.