| Features | | | New generation for efficient and quick RNA isolation from cells and tissues | • NucleoSpin® gDNA Removal Column – no time consuming rDNase digestion
• Convenient handling – lysate clearing and gDNA removal with one column in one step
• New efficient lysis buffer – no b-mercaptoethanol or TCEP necessary |
| | | Technology | Two column silica-membrane system
1. column for DNA removal
2. column for RNA isolation | | Format | Mini spin columns | | Sample material | < 1 x 107 cultured cells,
< 109 bacterial cells,
< 108 yeast cells,
< 30 mg human / animal tissue | | Fragment size | > 200 nt | | Typical yield | HeLa cells 5 x 106: 40-60 µg
Mouse liver 20 mg: 80-100 µg
Mouse kidney 20 mg: 40-70 µg
Mouse spleen 5 mg: 30-60 µg | | A260/A280 | 1.9–2.1 | | A260/A230 | 1.8-2.5 | | Typical RIN (RNA integrity number) | > 9 | | Elution volume | 30–120 µL | | Preparation time | 20 min/6 preps | | Binding capacity | 200 µg | | Applications* | | • RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
RNase protection assays | | * Kits to be used for research purposes only |
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