| Features |
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| New generation for efficient and quick RNA isolation from cells and tissues |
• NucleoSpin® gDNA Removal Column – no time consuming rDNase digestion
• Convenient handling – lysate clearing and gDNA removal with one column in one step
• New efficient lysis buffer – no b-mercaptoethanol or TCEP necessary |
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| Technology |
Two column silica-membrane system
1. column for DNA removal
2. column for RNA isolation |
| Format |
Mini spin columns |
| Sample material |
< 1 x 107 cultured cells,
< 109 bacterial cells,
< 108 yeast cells,
< 30 mg human / animal tissue |
| Fragment size |
> 200 nt |
| Typical yield |
HeLa cells 5 x 106: 40-60 µg
Mouse liver 20 mg: 80-100 µg
Mouse kidney 20 mg: 40-70 µg
Mouse spleen 5 mg: 30-60 µg |
| A260/A280 |
1.9–2.1 |
| A260/A230 |
1.8-2.5 |
| Typical RIN (RNA integrity number) |
> 9 |
| Elution volume |
30–120 µL |
| Preparation time |
20 min/6 preps |
| Binding capacity |
200 µg |
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Applications*
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• RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
RNase protection assays |
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* Kits to be used for research purposes only
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