| Features | | | Mini spin kit for the isolation of RNA of highest integrity |  • rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
• NucleoSpin® Filters (shredders) – efficient sample homogenization and reduction of viscosity
• Up to 70 μg ready-to-use RNA
• Parallel purification of genomic DNA by using the NucleoSpin® RNA/DNA Buffer Set• Also available for QIAcube® (please see “Ordering information” and contact Technical Service
at techbio@mn-net.com for more information). |
| | | Technology | Silica-membrane technology | | Format | Mini spin columns | | Sample material | < 5 x 106 cultured cells,
< 109 bacterial cells,
< 108 yeast cells,
< 30 mg human / animal tissue | | Fragment size | > 200 nt | | Typical yield | 14 μg (106 HeLa cells), 70 μg (109 bacterial cells) | | A260/A280 | 1.9–2.1 | | Typical RIN (RNA integrity number) | > 9 | | Elution volume | 40–120 µL | | Preparation time | 35 min/6 preps | | Binding capacity | 200 µg | | Applications* | | • RNA isolation from cultured cells, tissue (standard protocol)
• RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
• RNA from ≤ 100 μL biological fluids
• RNA clean-up from reaction mixtures
• RNA from samples stored in RNAlater®
• RNA from saliva samples collected with Oragene®•RNA (Genotek)
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension,
array technology, RNase protection assays | | * Kits to be used for research purposes only |
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• rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
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