| Features |
|
| Mini spin kit for the isolation of RNA of highest integrity |
 • rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
• NucleoSpin® Filters (shredders) – efficient sample homogenization and reduction of viscosity
• Up to 70 μg ready-to-use RNA
• Parallel purification of genomic DNA by using the NucleoSpin® RNA/DNA Buffer Set
• Also available for QIAcube® (please see “Ordering information” and contact Technical Service
at techbio@mn-net.com for more information).
|
|
|
| Technology |
Silica-membrane technology |
| Format |
Mini spin columns |
| Sample material |
< 5 x 106 cultured cells,
< 109 bacterial cells,
< 108 yeast cells,
< 30 mg human / animal tissue |
| Fragment size |
> 200 nt |
| Typical yield |
14 μg (106 HeLa cells), 70 μg (109 bacterial cells) |
| A260/A280 |
1.9–2.1 |
| Typical RIN (RNA integrity number) |
> 9 |
| Elution volume |
40–120 µL |
| Preparation time |
35 min/6 preps |
| Binding capacity |
200 µg |
|
|
Applications*
|
|
• RNA isolation from cultured cells, tissue (standard protocol)
• RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
• RNA from ≤ 100 μL biological fluids
• RNA clean-up from reaction mixtures
• RNA from samples stored in RNAlater®
• RNA from saliva samples collected with Oragene®•RNA (Genotek)
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension,
array technology, RNase protection assays |
|
|
* Kits to be used for research purposes only
|
|
Avaliações
Não há avaliações ainda.