POLYMYXIN B SELECTIVE SUPPLEMENT

Description

Supplement contains antibiotics which are targetted to selective species in for isolation and identification of pathogenic Staphylococcus and Bacillus species in TM 1120 BACILLUS CEREUS AGAR BASE, TM 1207 KG AGAR BASE, TM 851 SALT POLYMYXIN BROTH BASE, TM 197 MYP AGAR BASE, TM 1255

Categoria:

MODIFIED MYP AGAR BASE alongwith 50ml Egg Yolk Emulsion (TS 002) or to 200ml TM 851 SALT POLYMYXIN BROTH BASE.

 

Composition

Ingredients                                mg/500ml (per vial)

Polymyxin B Sulphate            50,000 units

(pe vial, sufficient for 500 ml of sterilized medium)

   Storage

Vials should be stored in sealed container in cool, dry location and protected from direct sunlight.

 

  Instructions  for Use

Aseptically rehydrate the vial content into 2ml of sterile distilled water. Mix well and add into sterile molten medium when cooled to 500C and dispense as desired. Use the medium according to the manufacturer’s recommendation. Store the prepared media plates inverted at 4-8°C for not more than 7 days.

 

  Microbiological Tests

The following results were obtained on inoculating different culture strains (103CFU/ml) at an incubation temperature of 35 – 370C for 18 – 48 hours in Bacillus Cereus Agar Base (TM 1120) KG Agar Base (TM      1207) Salt Polymyxin Broth Base (TM 851) or to 450ml of MYP Agar Base (TM 197) / Modified MYP Agar Base (TM 1255) alongwith 50ml  Egg Yolk Emulsion (TS 002) or to 200ml  Salt Polymyxin Broth Base (TM  851).

 

Microorganisms

ATCC

Inoculum (CFU/ml)

Growth

Appearance of colony

Lecithinase activity

(+ = haloes around the colonies)

Bacillus subtilis

6633

103

Good

Yellow

Negative

Bacillus cereus

10876

103

Good

Red

Positive

Staphylococcus aureus

25923

103

Good

Yellow

Positive

Escherichia coli

25922

103

Fair

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