The rDNase Set is designed for use with NucleoSpin RNA Kits. It is suitable for time saving on-column DNA removal, as well as for most efficient DNA removal in the eluate. Furthermore, the rDNase Set is suitable for digestion of contaminating DNA within pre-purified RNA preparations (e.g. phenol based RNA preparations), i.e. for DNA digestion in solution.
|Samples of 100 µl crude RNA solution, contaminated with DNA, were treated with rDNase according to the protocol. Subsequently, the RNA was isolated using NucleoSpin RNA Clean-up XS.
For the detection and determination of DNA and possible residual DNA, a qPCR reaction was performed before and after rDNAse treatment and clean-up. Results are shown in figure 1.Analysis of RNA quality and quantity was performed using a Bioanalyzer and RNA 6000 Nano Reagent and chip (Agilent). Data of RNA integrity before and after rDNase digestion and clean-up are presented in figure 2.
Efficient DNA removal from crude RNA extracts
qPCR was performed before and after rDNase treatment of 3 samples (81 bp beta-Globin target; DyNamo Capillary SYBR® Green Kit (Finnzymes #F-420S/L)).
DNA contaminations are efficiently removed by rDNase digestion, resulting in Ct values, higher 35.
|Unchanged high RNA integrity after rDNase digestion
RNA quality and quantity of the same sample were analyzed with an Agilent Bioanalyzer, Agilent RNA 6000 Nano chip, and Agilent RNA 6000 Nano reagent before and after rDNase digestion and clean-up.
MACHEREY-NAGEL rDNase shows high specificity.
RNA integrity is unaffected by rDNase treatment and RIN remains unchanged, before and after rDNase digestion.
*Increase of concentration is an effect of the RNA
clean-up procedure with NucleoSpin RNA Clean-up XS