YEAST EXTRACT-R (Bacto Grade) – 500 GM

Ref. No.: TBL/QA/STS/MB/73

STANDARD TESTING SPECIFICATION

For

YEAST EXTRACT POWDER-R

( BACTERIOLOGICAL GRADE) CODE –1532

Rev. No. : 00

Issue No. : 01

Date : 01/08/2015

Date : 01/09/2012

Page No. : 01 of 01

 

Categoria:

PHYSICAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Appearance

Yellow to light brownish free flowing powder having characteristic

yeast odour but not pungent smell.

2.

Solubility (2% soln. at 25oC)

Soluble in distilled water, clear. Insoluble in alcohol

3.

Clarity (2% Soln. at 121ºC.)*

Clear solution. No ppt.

* –After Autoclave

 

CHEMICAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

pH (2% Soln. at 25ºC)

5.5 – 6.5

2.

Loss on drying (at 105ºC)

NMT-  6.0%

3.

Total Nitrogen ( DWB)

NLT-  10.0%

4.

α – Amino Nitrogen

NLT-  3.0%

5.

Total Ash

NMT- 15.0%

6.

Chloride (as NaCl)

NMT- 5.0%

7.

Indole Test

Positive

MICROBIAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Total viable count

NMT- 10,000cfu/gm

2.

Escherichia coli

Negative

3.

Salmonella spp.

Negative

4.

Staphylococcus aureus

Negative

5.

Yeast & molds

100/gm

GROWTH PROMOTION TEST

 

Cultural Characteristic observed in 2%Yeast Extract-R and 1.5% agar after incubation at 35-37°C for 18-24 hours.

S.L. No.

MICROORGANISMS

ATCC

GROWTH

1.

Staphylococcus aureus

25923

Good-Luxuriant

2.

Escherichia coli

25922

Good-Luxuriant

3.

Pseudomonas aeruginosa

27853

Good-Luxuriant

4.

Bacillus subtilis

6633

Good-Luxuriant

5.

Enterococcus faecalis

29212

Good-Luxuriant

6.

Streptococcus pyogenes

19615

Good-Luxuriant

7.

Lactobacillus casei

9595

Good-Luxuriant

Best Before             : 36 Months

Std. Packing           : 25kg, HDPE drum

Storage                   : Hygroscopic by nature, store the material below 25ºC, protect from direct sunlight.

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